sirna loading (Santa Cruz Biotechnology)
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93
Structured Review
Santa Cruz Biotechnology
sirna loading
Sirna Loading, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pai+1+sirna/PAI-1+siRNA/pmc12579650-335-1-4
Average 93 stars, based on 25 article reviews
Sirna Loading, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pai+1+sirna/PAI-1+siRNA/pmc12579650-335-1-4
Average 93 stars, based on 25 article reviews
sirna loading - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "Developing Nanoparticle-Based Therapeutics for Targeting the Microenvironment in Obesity-Induced Endometrial Cancer"
Article Title: Developing Nanoparticle-Based Therapeutics for Targeting the Microenvironment in Obesity-Induced Endometrial Cancer
Journal: Cellular and Molecular Bioengineering
doi: 10.1007/s12195-025-00853-2
Figure Legend Snippet: Endometrial tumor microenvironment secreted factors induce an ASC phenotype with elevated expression of ITGA7 and PAI-1. A ITGA7 and B PAI-1 fold gene expression in ASCs followed by treatment with KLE and EME6/7 conditioned media (CM). C PAI-1 gene expression in ASC treated with PAI-1 siRNA before exposure to KLE conditioned media (KLE), blocks the PAI-1 increase observed after endometrial exposure. D Indirect co-culture system for ASC and KLE or ASC and EME6/7. E Gene expression of ITGA7 and F PAI-1 in ASC after 48 h of indirect co-culture. Values are means ± standard errors, from one-way ANOVA analysis followed with Tukey’s multiple comparisons test. * p = 0.05; ** p < 0.01; **** p < 0.0001; n = 3 biological replicates with three technical replicates per group
Techniques Used: Expressing, Gene Expression, Co-Culture Assay
Figure Legend Snippet: Fabrication and Characterization of nanoparticles. A fabrication of the LCMSN construct depicting the siRNA loading in the MSN core, liposomes, ITGA7 antibody conjugation and their fusion into the final construct ITGA7-LCMSN schematic. B Hydrodynamic size, and zeta potential evolution of MSN, loaded MSN with lipids and complete LCMSN with ITGA7 antibody (AB). C TEM images of implemented MSN, D siRNA loading and release and E and F TEM of negatively stained particles upon AB conjugation. Scale bar = 100 nm
Techniques Used: Construct, Liposomes, Conjugation Assay, Zeta Potential Analyzer, Staining
Figure Legend Snippet: Whole body biodistribution and siRNA delivery efficiency of DyLight 633-labeled ITGA7-LCMSNs loaded with PAI-1 siRNA after IP injection. A KINO images of mice injected intraperitoneally with Dy633 ITGA7-LCMSN and untargeted LCMSN, B ex vivo imaging of uterus at t = 24 h showing nanoparticle retention. C ex vivo KINO images of gonadal fat. D quantitative measurement of organ nanoparticle retention and E uterus nanoparticle fluorescence after 48 h of IP injection. F PAI-1 and ITGA7 gene expression in the uterus after in vivo IP injection of ITGA7-LCMSNs loaded with PAI-1 siRNA. G Endometrial PAI-1 gene expression in ob/ob and lean (WT) animals, as well as ob/ob animals treated with targeted (ITGA7-LCMSN) and untargeted (LCMSN) nanoparticles loaded with PAI-1 siRNA. Values are means ± standard errors, from unpaired t test. ** p < 0.001; **** p < 0.0001; n = 3 animals per group
Techniques Used: Labeling, Injection, Ex Vivo, Imaging, Fluorescence, Gene Expression, In Vivo
Related Articles
Cell Culture:Article Title: Aging, Plasminogen Activator Inhibitor 1, Brain Cell Senescence, and Alzheimer’s Disease Article Snippet: The lentivirus was packaged in 293T cells and transduced into target cells as company’s instruction (System Biosciences, Palo Alto, CA USA). .. Primary human astrocytes (ScienCell Research Laboratories, Catlog No #1800, Carlsbad, CA USA), cultured with Astrocyte Medium (Catlog No #1801, Carlsbad, CA USA), were transfected with non-targeted (NT) siRNA or Transfection:Article Title: Aging, Plasminogen Activator Inhibitor 1, Brain Cell Senescence, and Alzheimer’s Disease Article Snippet: The lentivirus was packaged in 293T cells and transduced into target cells as company’s instruction (System Biosciences, Palo Alto, CA USA). .. Primary human astrocytes (ScienCell Research Laboratories, Catlog No #1800, Carlsbad, CA USA), cultured with Astrocyte Medium (Catlog No #1801, Carlsbad, CA USA), were transfected with non-targeted (NT) siRNA or Article Title: PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells Article Snippet: A549 cells were cultured with Ham’s F-12 medium (21127-022, GibCo, CA, USA) containing 10% FBS and antibiotics. .. The cells were transfected with non-target siRNA or Article Title: Plasminogen activator inhibitor 1, fibroblast apoptosis resistance, and aging-related susceptibility to lung fibrosis Article Snippet: Apoptotic cells were analyzed with flow cytometer at UAB Flow Cytometry facility. siRNA transfection Primary human lung fibroblasts (CCL-210 cells), purchased from American Type Culture Collection (ATCC, Manassas, VA), were seeded in 60 mm cell culture dish at the density of 4 × 10 5 cells/well. .. After reaching 60–80% confluence, cells were transfected with either Article Title: TM5441, a plasminogen activator inhibitor-1 inhibitor, protects against high fat diet-induced non-alcoholic fatty liver disease Article Snippet: .. For PAI-1 knockdown in HepG2 cells, after reaching 70–80% confluence, cells were seeded in 6-well plates with medium containing 20 nM Article Title: PAI-1 Regulation of p53 Expression and Senescence in Type II Alveolar Epithelial Cells. Article Snippet: A549 cells were cultured with Ham’s F-12 medium (21127-022, GibCo, CA, USA) containing 10% FBS and antibiotics. .. The cells were transfected with non-target siRNA or Control:Article Title: Plasminogen activator inhibitor 1, fibroblast apoptosis resistance, and aging-related susceptibility to lung fibrosis Article Snippet: Apoptotic cells were analyzed with flow cytometer at UAB Flow Cytometry facility. siRNA transfection Primary human lung fibroblasts (CCL-210 cells), purchased from American Type Culture Collection (ATCC, Manassas, VA), were seeded in 60 mm cell culture dish at the density of 4 × 10 5 cells/well. .. After reaching 60–80% confluence, cells were transfected with either Article Title: Role of plasminogen activator inhibitor-1 in glucocorticoid-induced muscle change in mice. Article Snippet: Glucocorticoid (GC) is widely used for the therapy of various diseases, such as inflammatory bowel disease and rheumatoid arthritis [1, 2].. However, long-term use of GC induces numerous adverse effects such as infection, diabetes, muscle wasting and osteoporosis, and these adverse effects largely limit clinical use of GC [3–5].. Muscle wasting is one of the serious adverse effects of GC [4]. Knockdown:Article Title: TM5441, a plasminogen activator inhibitor-1 inhibitor, protects against high fat diet-induced non-alcoholic fatty liver disease Article Snippet: .. For PAI-1 knockdown in HepG2 cells, after reaching 70–80% confluence, cells were seeded in 6-well plates with medium containing 20 nM Negative Control:Article Title: ACT001, a novel PAI-1 inhibitor, exerts synergistic effects in combination with cisplatin by inhibiting PI3K/AKT pathway in glioma. Article Snippet: Cell culture and transfection of siRNAs U118MG cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% foetal bovine serum (Gibco), 100 IU/mL penicillin and 0.1 mg/ mL streptomycin (HyClone) in a humidified incubator containing 5% CO2 at 37 °C. .. Article Title: ACT001, a novel PAI-1 inhibitor, exerts synergistic effects in combination with cisplatin by inhibiting PI3K/AKT pathway in glioma Article Snippet: U118MG cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% foetal bovine serum (Gibco), 100 IU/mL penicillin and 0.1 mg/mL streptomycin (HyClone) in a humidified incubator containing 5% CO 2 at 37 °C. .. |
